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Fta2000 Setup Constants

Each instrument has specific software constants that calibrate and align the mechanics. The specific values for your instrument are included on a CDROM sent with the instrument. FTA does not keep a copy of these values at the factory, so it is your responsibility to maintain this information.

General notes on setup constants:

  • The gear ratio is the number of stepper motor steps per unit distance moved. This is the scaling constant for the controller. It is determined by the internal construction of the motor and the external leadscrews or gears employed. It must be correct for distances to be calibrated.

  • The max rate is the fastest speed for a motor. Because the controller is digital, only specific values are available, but these are very accurate. You can enter any desired value but your value will be replaced with the actual digital value after the next move. If you attempt to go too fast, the motor may stall. You set the max rate faster than the default value at your own risk. If the motor has no specific speed setting elsewhere in the software, it will always run at its max rate.

  • The home offset is the distance the motor must move to go from the mechanical home position, determined by a sensor, and the logical origin of the coordinate system. It will vary with each instrument because the mechanical position of the home sensor can be set only approximately during construction.

Values You Must Adhere To

These parameters have only one correct value (default). They must be correct.

  • Gear ratios.
  • Radial stage home sensor position ("stage type").

Values You Should Adhere To

If you do not adhere to these defaults, you operate the instrument at your own risk and the axis may fail to move as expected. FTA does not support rates in excess of the defaults shown in this document.

  • Max rates.

Values You Must Determine Locally

If you do not have the original factory settings, you must determine these experimentally.

  • Home offsets.
  • +/- motion limits.

Data Tables

The following tablesdescribe the setup constants specific to the autosampler and the sample stage. This information will be helpful if you lose your original values and have to reconstruct them.

Autosampler Tab Setup For Fta2000's Delivered Before 1 June 2004

Variable Default Description

Carousel gear

240

This motor turns the autosampler carousel. It is one of three similar Oriental Vexta gearhead stepper motors in the instrument. The gear is the number of stepper motor steps per degree of carousel rotation.

Fta2000's are always individualy built with the same type motor in all three positions, although different instruments may have different motor types. The easiest one to view is located on the bottom of the upper circular plate of the autosampler. The Oriental part number is PK243A2A-SG18. If the gear constant is incorrect, the carousel will turn the wrong distance in moving from one vial location to the adjacent vial; you could, in theory, compensate for an incorrect gear and still have it Home to Vial 1 by adjusting the Carousel home offset, but an incremental vial-to-vial move would still be wrong.

Carousel home offset

-

The offset is the number of degrees of carousel rotation to move Tip 1 to Vial 1 from the carousel Home position. Positive is clockwise when viewed from above. There is no default value since every instrument is different. If you have no idea, start with 0 and slowly change the value until Tip 1 falls over Vial 1 after Home All. Use as small an absolute value as possible; e.g., use -30 rather than +330 degrees.

Carousel max rate

8.33

The turning rate for the carousel.

Carousel rescue move

0

This is used only to move the carousel during maintenance operations. A non-zero value causes the next Move Carousel command to move the carousel without regard to vial positions or direction. See Fta2000 Carousel Setup for further information.

Vial pitch

15

The angular distance between autosampler vials.

Vial count

17

The number of vial positions.

Distribution valve gear

144

The gear ratio for the Oriental Vexta gearhead motor driving the Hamilton distribution valve. The distribution valve is the 6-way valve that connects the Lee pump to individual dispense tips on the carousel. If this value is wrong, some tips may not receive liquid and others may receive it at the wrong time. The Hamilton valve is operated by the Move Valve command.

Distribution valve home offset

-

The offset is the number of degrees of valve rotation to move the valve outlet to the Tip 1 position from Home.

Distribution valve max rate

13.89

The turning rate for the distribution valve shaft.

Pump gear

40

The gear for the Lee pump located on the carousel.

Pump volume

50

The capacity of the Lee pump.

Pump max rate

10

The pump will mechanically run faster but will cause cavitation and the creation of air bubbles in the system liquid, which is bad.

Tip Z gear

629.92

The gear for the six Tip Z drives on the carousel. The linear actuator motor is located within the upper cap on the carousel. It is a Haydon Switch E26449-05.

Tip Z max rate

6.35

The operating rate for the tips, except in special circumstances. The tip motor is particularly sensitive to loading and handling. It is possible it will only operate at 4.23.

Tip Z slow rate

1

An alternative rate for certain special movements by the Sequencer.

Tip Z - motion limit

-95

Software limit on how far down the tip can go. If your tip will not go any lower, but appears to have leadscreww left, check this value.

Tip tray offset

-

Rotation of the tray from Home to Tip 1. This should be seen only if you have an automatic tip changer tray installed. If not, uncheck "Tip Changer" on the drop down list in Computer Controlled Hardware on the LogOn screen.

Tip pickup Z

-

The Tip Z down position to pickup tips.

Tip detach Z

-

The Tip Z down position to detach tips.

Autosampler Tab Setup For Fta2000's Delivered After 1 June 2004

Variable Default Description

Carousel gear

120

This motor turns the autosampler carousel. It is one of three similar Oriental Vexta gearhead stepper motors in the instrument. The gear is the number of stepper motor steps per degree of carousel rotation.

Fta2000's are always individualy built with the same type motor in all three positions, although different instruments may have different motor types. The easiest one to view is located on the bottom of the upper circular plate of the autosampler. The Oriental part number is PK243A2A-SG9. If the gear constant is incorrect, the carousel will turn the wrong distance in moving from one vial location to the adjacent vial; you could, in theory, compensate for an incorrect gear and still have it Home to Vial 1 by adjusting the Carousel home offset, but an incremental vial-to-vial move would still be wrong.

Carousel home offset

-

The offset is the number of degrees of carousel rotation to move Tip 1 to Vial 1 from the carousel Home position. Positive is clockwise when viewed from above. There is no default value since every instrument is different. If you have no idea, start with 0 and slowly change the value until Tip 1 falls over Vial 1 after Home All. Use as small an absolute value as possible; e.g., use -30 rather than +330 degrees.

Carousel max rate

16.7

The turning rate for the carousel.

Carousel rescue move

0

This is used only to move the carousel during maintenance operations. A non-zero value causes the next Move Carousel command to move the carousel without regard to vial positions or direction. See Fta2000 Carousel Setup for further information.

Vial pitch

15

The angular distance between autosampler vials.

Vial count

17

The number of vial positions.

Distribution valve gear

72

The gear ratio for the Oriental Vexta gearhead motor driving the Hamilton distribution valve. The distribution valve is the 6-way valve that connects the Lee pump to individual dispense tips on the carousel. If this value is wrong, some tips may not receive liquid and others may receive it at the wrong time. The Hamilton valve is operated by the Move Valve command.

Distribution valve home offset

-

The offset is the number of degrees of valve rotation to move the valve outlet to the Tip 1 position from Home.

Distribution valve max rate

27.78

The turning rate for the distribution valve shaft.

Pump gear

40

The gear for the Lee pump located on the carousel.

Pump volume

50

The capacity of the Lee pump.

Pump max rate

10

The pump will mechanically run faster but will cause cavitation and the creation of air bubbles in the system liquid, which is bad.

Tip Z gear

157.48

The gear for the six Tip Z drives on the carousel. The linear actuator motor is located within the upper cap on the carousel. It is a Haydon Switch E26441-05.

Tip Z max rate

12.7

The operating rate for the tips, except in special circumstances. The tip motor is particularly sensitive to loading and handling. It is possible it will only operate at 8.46.

Tip Z slow rate

1

An alternative rate for certain special movements by the Sequencer.

Tip Z - motion limit

-95

Software limit on how far down the tip can go. If your tip will not go any lower, but appears to have leadscreww left, check this value.

Tip tray offset

-

Rotation of the tray from Home to Tip 1. This should be seen only if you have an automatic tip changer tray installed. If not, uncheck "Tip Changer" on the drop down list in Computer Controlled Hardware on the LogOn screen.

Tip pickup Z

-

The Tip Z down position to pickup tips.

Tip detach Z

-

The Tip Z down position to detach tips.

R-Theta-Z-Tilt Stage Tab Setup For Fta2000's Delivered Before 1 June 2004

Variable Default Description

R gear

157.48

The radial stage gear.

R home offset

-

Typically a few millimeters so the final radial position after Home is as close to the end of the carriage way as possible without touching.

R max rate

8.46

The operating rate for the stage.

R - motion limit

-

Software limit on motion in negative direction. Typically a few millimeters negative, but adjust to suit your stage.

R + motion limit

-

Software limit on motion in the positive direction. Typically about 150 millimeters, but adjust to suit your stage.

R stage setup

0

Determines which end of the radial stage contains the home sensor. Use 0 when the home sensor is at the end closest to the camera. These systems were normally type 0.

Theta gear

240

The rotary stage gear determined by the Oriental Vexta gearhead motor.

Theta home offset

-

Angular distance from Home to the desired starting location on the stage. This is arbitrary and may be set anywhere that is convenient.

Theta max rate

8.33

The operating rate for the rotation stage.

Z gear

157.48

The Z stage gear.

Z home offset

-

Typically a few millimeters positive. This may be set at any convenient value but it obviously affects all subsequent Z positions in the Sequencer.

Z max rate

2.12

The operating rate for the Z axis. The axis will go faster under no load but is strongly affected by the weight of the platter and must be adjusted carefully.

Z - motion limit

-

Software imposed limit on Z motion. If your stage stops at a height which seems unreasonable, check this parameter.

Z + motion limit

-

Software imposed limit on Z motion. If your stage stops at a height which seems unreasonable, check this parameter.

Tilt gear

616

The gear for the tilt stage.

Tilt home offset

-

The distance from Home to a level stage.

Tilt max rate

1.082

You can set a slower operational rate in the software.

Tilt - motion limit

-

Software imposed limit on tilt motion. If your stage stops at an angle which seems unreasonable, check this parameter.

Tilt + motion limit

-

Software imposed limit on tilt motion. If your stage stops at an angle which seems unreasonable, check this parameter.

R-Theta-Z-Tilt Stage Tab Setup For Fta2000's Delivered After 1 June 2004

Variable Default Description

R gear

157.48

The radial stage gear.

R home offset

-

Typically a few millimeters so the final radial position after Home is as close to the end of the carriage way as possible without touching.

R max rate

8.46

The operating rate for the stage.

R - motion limit

-8

Software limit on motion in negative direction. Typically a few millimeters negative, but adjust to suit your stage.

R + motion limit

150

Software limit on motion in the positive direction. Typically about 150 millimeters, but adjust to suit your stage.

R stage setup

1

Determines which end of the radial stage contains the home sensor. Use 1 when the home sensor is at the end farthest from the camera. These systems are type 1.

Theta gear

120

The rotary stage gear determined by the Oriental Vexta gearhead motor.

Theta home offset

-

Angular distance from Home to the desired starting location on the stage. This is arbitrary and may be set anywhere that is convenient.

Theta max rate

16.67

The operating rate for the rotation stage.

Z gear

157.48

The Z stage gear.

Z home offset

-

Typically a few millimeters positive. This may be set at any convenient value but it obviously affects all subsequent Z positions in the Sequencer.

Z max rate

2.12

The operating rate for the Z axis. The axis will go faster under no load but is strongly affected by the weight of the platter and must be adjusted carefully.

Z - motion limit

-

Software imposed limit on Z motion. If your stage stops at a height which seems unreasonable, check this parameter.

Z + motion limit

-

Software imposed limit on Z motion. If your stage stops at a height which seems unreasonable, check this parameter.

Tilt gear

616

The gear for the tilt stage.

Tilt home offset

-

The distance from Home to a level stage.

Tilt max rate

1.082

You can set a slower operational rate in the software.

Tilt - motion limit

-5

Software imposed limit on tilt motion. If your stage stops at an angle which seems unreasonable, check this parameter.

Tilt + motion limit

90

Software imposed limit on tilt motion. If your stage stops at an angle which seems unreasonable, check this parameter.